|
KMID : 0616120210520030186
|
|
Korean Journal of Pharmacognosy
2021 Volume.52 No. 3 p.186 ~ p.191
|
|
|
HPLC/DAD Method Validation of 6-Hydroxyluteolin 7-O-Glucoside Analysis from Salvia plebeia
|
|
Lee Hak-Dong
Paje Leo Adrianne
Choi Jung-Won
Kim Ju-Ree
Yu A-Ram
Bae Min-Jung
Lee Sang-Hyun
|
|
|
Abstract
|
|
|
|
We developed a method to identify and quantify 6-hydroxyluteolin 7-O-glucoside in the powder of Salvia plebeia (PS) using high-performance liquid chromatography coupled with diode array detector (HPLC/DAD) and equipped with reverse-phase INNO C18 column. The analytical method was optimized and validated using novel parameters. The obtained values for the limits of detection and quantification were 3.60 and 10.90 μg/mL, respectively. Calibration curve showed good linearity in the concentration range tested (0.00625-0.1 mg/mL, r2 = 1.0000), high accuracy (96.2-101.4%), and precision values (RSD ￡ 0.27%). Our analysis support the use of our method for accurately identifying and quantifying 6-hydroxyluteolin 7-O-glucoside from PS in routine analyses and large-scale extraction processes for content determination.
|
|
|
KEYWORD
|
|
|
Salvia plebeia, 6-Hydroxyluteolin 7-O-glucoside, Method validation, HPLC/DAD
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|